Single-shot off-axis FF-OCT (SO-FF-OCT) as a multimodal contrast system: Doppler and dynamic contrasts

The last few decades have seen the emergence of a huge number of optical imaging techniques. Among these, full-field optical coherence tomography (FF-OCT) has become valuable for many biomedical applications. Indeed, FF-OCT is a noninvasive and label-free imaging technique that produces high-resolution 3D microscopic images of ex-vivo scattering biological samples without the need for labels. We have developed a single-shot off-axis FF-OCT (SO-FF-OCT) approach that extends these capabilities to in-vivo imaging, enabling the observation of cellular-scale structures in living samples. This advancement is particularly significant for observing active vascularization and cellular activity within living tissues. In this work, we present the first results of Doppler and dynamic imaging using the SO-FF-OCT system, showcasing its potential for real-time, high-resolution imaging in living organisms.