Realtime optical coherence tomography monitoring suprachoroidal injection in living rabbits

Suprachoroidal injections (SCI) allow for precise delivery of therapeutic agents to the posterior segment of the eye, targeting specific retinal layers and structures affected by diseases such as macular edema and choroidal neovascularization. This study investigates real-time optical coherence tomography (OCT) monitoring of SCI using a cost-effective customized needle adapted from a commercial insulin needle, which reduces costs significantly compared to specialized suprachoroidal needles. Real-time OCT imaging revealed gradual detachment of the suprachoroidal layer with accumulation of the injected fluid. The injection bleb rapidly diminished and resolved within 20 minutes post-injection. This approach not only enhances the understanding of injection dynamics but also offers potential applications in optimizing therapies for retinal diseases with improved efficacy and reduced procedural costs.