Noninvasive evaluation of mouse embryo quality using time-lapse bright field and optical coherence microscopy

The selection of high-quality embryos is essential to enhance the implantation rate for in vitro fertilization (IVF). Optical coherence microscopy (OCM) can provide 3D label-free high-resolution imaging of embryos. The microstructures can assist in embryo evaluation and understanding embryo development. Here, we acquired time-lapse 3D OCM images with co-registered bright-field imaging on mouse embryos from the one-cell stage to blastocysts inside an incubator, demonstrating the structural features of the embryos. The second and third embryo’s cell cycle suggested a relationship between blastocyst formation and the hatching capability. OCM-based time-lapse technology is promising to enrich insights and help embryo selection for IVF.